Особенности внутриклеточного транспорта малых неструктурных белков вирусов растений, определяющих вирулентность и взаимодействие с хозяином

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1. Показано, что белок 1Л-ОРТ6 вируса табачной мозаики, но не белок Ь-ОРТ6 вируса томатной мозаики, значительно усиливает патогенность рекомбинантного вируса погремковости табака в растениях МсоНапа ЬеШкттапа.

2. С-концевой район 1Л-ОРТ6 и Ь-ОРТ6 определяет влияние белка на патогенность вируса.

3. Белок Ш-ОРТ6 на ранних этапах экспрессии в клетке локализуется в ядрышке, а затем перемещается в митохондрии, тогда как белок Ь-ОРТб обнаруживается в эндоплазматическом ретикулуме, не меняя субклеточной локализации.

4. Белки Ш-ОРТ6 и Ь-ОРТ6 несут сигнал ядрышковой локализации в М-концевом районе. С-концевой район Ь-ОРТ6 необходим для взаимодействия белка с мембранами эндоплазматического ретикулума.

5. Показано, что единичная замена в составе аминокислотной последовательности белка Ь-ОРТ6, блокирующая образование комплекса с фактором элонгации трансляции еЕР1 А, нарушает также кооперативность связывания белка с нуклеиновыми кислотами.

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Содержание

СПИСОК СОКРАЩЕНИЙ.

I ОБЗОР ЛИТЕРАТУРЫ.

Ядерно-цитоплазматический транспорт.

Общая характеристика ядра и ядерных структур.

Процесс транспорта через ядерную пору.

Сигналы ядерной локализации.

Сигналы ядерного экспорта.

Сигналы ядрышковой локализации.

Взаимодействие белков РНК- вирусов растений и вироидов с ядерными белками и структурами.

РНК вирусы с геномом положительной полярности.

Вироиды.

Тобамовирусы.

II МАТЕРИАЛЫ И МЕТОДЫ.

III РЕЗУЛЬТАТЫ.

Мутация инициаторного кодона ОРТ6 не влияет на патогенность ВТоМ.

Влияние белков L- и U1-OPT6 и их гибридов на патогенез ВПТ.

Субклеточная локализация L- и U1-OPT6 и их гибридов.

Детерминанты субклеточной локализации L- и U1-OPT6.

Изучение взаимодействия между L-OPT6 и eEFIA in vitro.

Аминокислотная замена I11T в L-OPT6 препятствует образованию комплекса с eEFIA и кооперативному связыванию нуклеиновых кислот.

IV ОБСУЖДЕНИЕ РЕЗУЛЬТАТОВ.

V ВЫВОДЫ.

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